Fig. 2
From: A radiosensitizing effect of RAD51 inhibition in glioblastoma stem-like cells
Expression of DNA repair genes in GSCs after IR. a TLDA expression levels of the most significant DNA repair genes. Relative expressions were measured 3 h following IR, data represent the mean ± SD of 10 GSCs determined by 2-ΔΔCt quantification method. Relative expressions of target genes were determined using GAPDH as endogenous control (**p < 0.01, *p < 0.05). b mRNA expression of RAD51, BRCA1, BRCA2, CHK1 and CHK2 in group 1 and group 2. The vertical scatter plot shows the log10 expression of relative quantification (RQ) values normalized to the expression before IR. Each data point represents one GSC line measured in triplicate (*p = 0.032). c Western blot analysis of RAD51 following 4Gy and 12Gy IR. Total protein were extracted after 45 min and 24 h following IR, β-actin was used as loading control. Densitometric analysis of specific signals shows relative RAD51 protein expression levels normalized with β-actin and expressed as a percentage of control in GSC-6 and GSC-11 (n = 3) (*p < 0.05) (Image J software)