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Fig. 3 | BMC Cancer

Fig. 3

From: Identification of epigenetic factors regulating the mesenchyme to epithelium transition by RNA interference screening in breast cancer cells

Fig. 3

High throughput functional screen to detect genes potentially involved in MET. a Four examples obtained in the initial screen. E-CAD expression was normalized to cell number then data were normalized to the median of SMARTpools in the same plate (n = 80 SMARTpools/plate). Threshold and hits selection were based on MAD calculation. b Morphological changes are revealed by F-ACTIN staining (red) as described before. Scale bars, 100 μm

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