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Fig. 3 | BMC Cancer

Fig. 3

From: Photodynamic therapy combined to cisplatin potentiates cell death responses of cervical cancer cells

Fig. 3

Cell death profile induced by each treatment, according to cell line. Cell lines C-33 A (panel a), HaCaT (panel b) and SiHa (panel c) were submmited to Hoechst 33342, propidium iodide and fluorescein diacetate staining after treatments (CUR [25 μM for 6 h]; DOX [50 μg/mL for 6 h]; CisPT [41.6 μM for 6 h]; MB [19.5 μM for 20 min]; LED 660 nm [5.11 J/cm2]; PG [0.5 μM for 2 h]; LED 630 nm [2.76 J/cm2]; MBPDT [19.5 μM MB + 5.11 J/cm2 LED 660 nm]; MBPDT + CisPT [19.5 μM MB + 5.11 J/cm2 LED 660 nm + 1.3 μM CisPT for 6 h]; PGPDT [0.5 μM PG + 2.76 J/cm2 LED 630 nm]; CisPT + PGPDT [1.3 μM CisPT for 6 h + 0.5 μM PG + 2.76 J/cm2 LED 630 nm]). Columns represent the average of three independent assays and bars represent standard deviation. Asterisks indicate the statistical difference, relative to negative control. Kruskal-Wallis, with Dunn’s post-hoc. *p < 0,05; **p < 0,01; ***p < 0,001. NT: non-treated; CUR: curcumin; DOX: doxorubicin; CisPT: cisplatin; MB: methylene blue; MBPDT: photodynamic therapy mediated by MB; PG: Photogem; PGPDT: photodynamic therapy mediated by PG

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