Fig. 5
From: Photodynamic therapy combined to cisplatin potentiates cell death responses of cervical cancer cells
Clonogenic survival. a Survival fraction of cell lines treated or not with MBPDT or MBPDT + CisPT. Columns represent the average number of colonies in each condition (a colony was considered so when presenting more than 50 cells), after three independent assays. Bars indicate the standard deviation. Asterisks indicate the statistical difference, relative to negative control. Kruskal-Wallis, with Dunn’s posthoc (*p < 0,05; **p < 0,01; ***p < 0,001). b Representative images of colonies formed in each treatment condition. Cristal violet staining; 10X objective (Olympus CKX31 microscope). NT: non-treated; MBPDT: photodynamic therapy mediated by methylene blue (19.5 μM MB + 5.11 J/cm2 LED 660 nm) MBPDT + CisPT: MBPDT followed by cisplatin treatment for 6 h (1.3 μM CisPT)