Fig. 3
Comparison of the differences in the mRNA levels associated with MVs and EXOs. a Non-supervised hierarchical clustering indicating normalized enrichment of the mRNA levels of the 46 most significant genes detected in the LNCaP and PC-3 MVs and EXOs in comparison with the PNT2 MVs or PNT2 EXOs. Volcano plots indicating changes in mRNA levels between: (b) LNCaP MVs vs PNT2 MVs; (c) LNCaP EXOs vs PNT2 EXOs; (d) PC-3 MVs vs PNT2 MVs; and (e) PC-3 EXOs vs PNT2 EXOs. Data is representative of three independent experiments per group. Data is reported as x-axis = log2 (fold change of sample/Ctrl), y-axis = negative log10 (P value). The horizontal blue line indicates a P value of 0.05. The vertical grey line indicates an absolute fold-change of 10. Red and green dots indicate increased or decreased mRNA levels of the genes of study in the MVs and EXOs in comparison to the PNT2 MVs and EXOs