Fig. 3

CCNJ was a novel target gene of miR-146a. a The binding site of miR-146a and the prediction target genes (CCNJ) through Targetscan web. b The wild type (CCNJ 3′-UTR-WT) or mutant (CCNJ 3′-UTR-MUT) reporter plasmids were co-transfected into 293T cells with miR-146a or NC lentivirus. The normalized luciferase activity in the control group was set as relative luciferase activity. c The mRNA levels of CCNJ in A549/DDP and SPC-A1/DDP cells stably transfected with miR-146a or NC lentivirus and incubated with 1 μg/ml DDP for 48 h were analyzed by RT-qPCR. d The protein levels of CCNJ, P-gp, MRP1, LRP, P53 and cleaved Caspase-3 were analyzed in A549/DDP and SPC-A1/DDP cells stably transfected with miR-146a or NC lentivirus by Western blot. All data represented as mean value ± SD from 3 independent experiments. N.S. = no significant, **p < 0.01, ***p < 0.05