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Fig. 5 | BMC Cancer

Fig. 5

From: Wogonin as a targeted therapeutic agent for EBV (+) lymphoma cells involved in LMP1/NF-κB/miR-155/PU.1 pathway

Fig. 5

Wogonin down-regulates the protein expression of NF-κB. a Effect of miR-155 inhibitor on p65 and pp65 protein expression. Raji cells were treated with 100nmol/L inhibitor for 48 h. The protein expression was analyzed by western blots. GAPDH was used as a loading control. b Effect of wogonin, PDTC and wogonin + PDTC on p65 and pp65 protein expression. Raji cells were treated with 50μmol/L wogonin, 50μmol/L PDTC or their combination respectively for 48 h. The protein expression was analyzed by western blots. GAPDH was used as a loading control. c The effects of miR-155 inhibitor, wogonin and PDTC on NF-κB expression showed the clear suppressive effect of wogonin and PDTC, while miR-155 inhibitor had little effect on activation of NF-κB. Raji cells were incubated with 100nmol/L miR-155 inhibitor, 50μmol/L PDTC or 50μmol/L wogonin respectively for 48 h, and then analyzed for NF-κB expression by EMSA. The comparisons were made relative to control group using intensity of protein. All the images were detected by Image J. Bars were the mean ± SD(n = 3). The different levels of significance was indicated as (*P < 0.05 and **P < 0.01)

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