Fig. 6From: MASTL inhibition promotes mitotic catastrophe through PP2A activation to inhibit cancer growth and radioresistance in breast cancer cellsMASTL depletion increases the radiosensitivity of breast cancer cells. MCF7 cells were transfected with either 5 nmol/l control siRNA or MASTL.5 siRNA. Cells were treated 0, 3 or 4 Gy irradiation for 42 h. a, b Cell proliferation was determined by FACS analysis and then analyzed by immunoblotted with the indicated antibodies. c The results of the clonogenic assay. Representative images of the cells treated the indicated conditions (left panel). The number of colonies was measured (right panel). d The sphere forming assay was performed (> 110 sphere colonies per data point). Scale bar = 100 μm. Representative images of sphere forming assay. e PP2A-Aα/β proteins were immunoprecipitated using an anti-PP2A-Aα/β antibody and analyzed for PP2A activity. The data represent typical results and are presented as the mean ± standard deviation of three independent experiments; **P < 0.01 and *P < 0.05Back to article page