Fig. 1

Expression of MAFb gene and protein in primary plasma cells and HMCLs. Affymetrix expression of MAFb mRNA was high in the GEP MF subgroup (a). Gene expression of 803 newly diagnosed MM patients was measured by U133 plus2.0 Affymetrix oligonucleotide microarray probe set 218559_s_at. The box plots indicate the middle of GEP signaling and dot lines with bars in black indicate quartiles. The cells were cultured in the growth mediate for 48 h and harvested for isolated RNA and MAFb mRNA in 27 HMCLs by qRT-PCR analysis as described in Material and methods (b). The cells were cultured in the growth mediate for 48 h and MAFb protein was measured in HMCLs by immunoblotting analysis described in Materials and Methods (c). MAFb protein in nuclei and cytoplasm of HMCLs (d) and MM PC from 4 patients (e) was determined with immunofluorescence staining with DAPI counterstaining. Images were taken with a fluorescence microscope with digital camera as described in supplemental data