Fig. 6
From: MAFb protein confers intrinsic resistance to proteasome inhibitors in multiple myeloma
Silencing MAFb enhanced PIs-induced apoptosis and activation of caspases. SACHI/shMAFb or SACHI/shCon cells were treated with serial concentrations of Bzb (a) or CFZ (b and c) with (c) or without HBMSC (a and b) for 14 h and apoptotic cell numbers determined by Annexin V staining and flow cytometry analysis as described in supplemental data. Results are presented as mean ± SE (n = 3). *P < 0 .01 versus control. **P < 0 .001 versus control. SACHI/shMAFb and SACHI/shCon cells were treated with indicated concentrations of Bzb (A) or CFZ (b) for14 hours. Protein was resolved in SDS-PAGE. The full-length (FL) and cleavage fragments (CFs) of each indicated protein were determined by immunoblotting analysis using antibodies specifically recognizing of caspases-3, − 7, − 8 and − 9, PARP and lamin A/C