Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: Investigation of the key chemical structures involved in the anticancer activity of disulfiram in A549 non-small cell lung cancer cell line

Fig. 2

Cytotoxic effect of DS and related compounds on A549 NSCLC cell line. a The chemical structures of DS-related compounds used in this study. b Viability curves of A549 cells in different treatments. The cytotoxicity of different compounds in monolayer-cultured A549 cells was examined. All the treatments except Cu-DDC were supplemented with CuCl2 (in a consistent concentration of 10μM). The cells were dosed for 72 h and then subjected to MTT assay. c IC50 (nM) values. d The morphology of the cells subjected to different treatments. The cells were treated with S-Me-DDC (10 μM), Cu-DDC (5μM), DDC (1μM), TMDS (1μM), DS (1μM) with or without Cu (10 μM) for 6 h and then release in drug-free medium overnight. The microscopic images were taken at 40× magnification. e and f Annexin V analysis of apoptosis. The cells were exposed to different compounds at the above concentrations with or without 10 μM of CuCl2 for 6 h and immediately subjected to Annexin V analysis. g Western blotting detection of the expression of apoptosis related proteins. The cells were subjected the same treatments as D before western blotting analysis. N = 3, * p < 0.05, ** p < 0.01

Back to article page