Fig. 2

C19 inhibits the growth of human colorectal cancer cell lines. a The colorectal cancer cell lines HCT116, LoVo and the normal colon cell line CCD-18Co were treated with the indicated concentrations of C19 for 72 h. IC₅₀ values of HCT116, LoVo and CCD-18Co cells were 103.03, 22.7 and 60.8 μM, respectively. b Treatment with C19 at a concentration of 10 μM significantly reduces the viability of the LoVo cell line. *p < 0.01 (c) Cells were treated with vehicle (DMSO), the IC₅₀ of C19 and IC₈₅ of 5-Fu in a clonogenic assay and stained with crystal violet. A representative plate of three independent experiments is shown. d Quantification of crystal violet staining from cells depicted in (c). n = 3; *** p < 0.0001