Table 1 Primer sequences for amplication of genes involved in cancer mechanism and GAPDH internal standard
From: Anti-cancer effect of dung beetle glycosaminoglycans on melanoma
Gene Symbol | Gene Name | Primer Sequence |
|---|---|---|
ACE1 | Angiotensin converting enzyme 1 | 5′-ACAGGCTCCATCCATCCTCC-3’ |
5′-TGGGGACAGCAAAGGAGGAA-3’ | ||
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 5’-GTGGAGATTGTTGCCATCAACGA-3’ |
5’-CCCATTCTCGGCCTTGACTGT-3’ | ||
GPX1 | Gluthathione peroxidase 1 | 5′-CCAACACCCAGTGACGACC-3’ |
5′-CTCAAAGTTCCAGGCAATGTC-3′ | ||
Hpse | Heparanase | 5′-ACTTGAAGGTACCGCCTCCG-3′ |
5′-GAAGCTCTGGAACTCGGCAA-3′ | ||
Protein tyrosine phosphatase, S | Protein tyrosine phosphatase, receptor type, S | 5′-CAAGCTGCCCCTCTCTCTCT-3’ |
5′-GCAGAAAGATCACACTGGCCC-3’ |