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Fig. 4 | BMC Cancer

Fig. 4

From: Drug resistance profiling of a new triple negative breast cancer patient-derived xenograft model

Fig. 4

Chemosensitivity profiles of TU-BcX-2 K1 amongst specific drug classes and targeted small molecule inhibitors. TU-BcX-2 K1 cells were treated for 72 h with the NCI oncology drug set (1 μM) or DMSO controls. Cells were harvested, stained with Calcein-AM and EthD III and fluorescence used to visualize the live and dead cells. Green = Calcein-AM (live cells), Red = EthD III (dead cells). a Representative images of stained TU-BcX-2 K1 cells showing varying therapeutic responses of TU-BcX-2 K1 cells to specific drugs within the cereblon inhibitors drug class. b Small molecule targeted inhibitors within the same drug class had different efficacy on TU-BcX-2 K1 cells. c Small molecule kinase inhibitors from the NCI oncology screen that reverse the mesenchymal morphology of TU-BcX-2 K1 cells included cirzotinib and vorinostat. All images were captured with fluorescence microscopy at 100X magnification. d Quantification of live and dead cells from the cereblon inhibitors drug class in panel A. Data is represented as total number of live compared to total number of dead cells. Significance was obtained by comparing numbers of live and dead cells in the treatment groups to respective DMSO controls. The symbol ‘*’ represents significance for live cell comparisons, and ‘ф’ shows significance for dead cell comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001

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