Fig. 7

TSG101expression in SK-MEL-28 cell. (A) Untreated SK-MEL-28 cells expressed TSG101 (green) marker in their cytoplasm (PhC- phase contrast) localized nearby nucleus (DAPI, blue). Cell nuclei are stained with DAPI (blue) and the last figure in column is overlaid (DAPI/TSG101/PhC); (B) Paclitaxel (PTX) treated SK-MEL-28 after 48 h. Cell nuclei are stained with DAPI (blue), cells are captured in phase contrast (PhC) and overlaid (DAPI/TSG101/PhC). After PTX treatment microcell formation was induced (DAPI, blue), nevertheless TSG101 (green) is not expressed in microcells but nearby nuclei. The scale bar is 20 μm