Figure 4

Endogenous Snail and E-cadherin expression in 10 HCC cell lines and a normal liver cell line (THLE-3). Endogenous expression levels of Snail and E-cadherin were measured by real-time qRT-PCR before siTPM transfection. Total RNA extraction and qRT-PCR procedure were as described in Materials and Methods. Human GAPDH gene was used as internal control for qRT-PCR. X axis represents cell lines and Y axis represents relative expression ratios of each gene (Cell lines/THLE-3). Error bars represent mean ± standard error of mean. Open bar represents Snail expression and closed bar represents E-cadherin expression.