Figure 6

Cytokines secreted by Mφ, by MDA-MB-231 cells and by the coculture. The assay was performed after 5 days of culture in the absence (A) or presence (B) of 5 μM TM (final concentration), the corresponding medium being renewed daily. Culture supernatants corresponding to the final 24 h of incubation were then collected and assayed for cytokine production using the Human Cytokine Antibody Array III kit (RayBiotech). Ellipses indicate the coordinates of the secreted cytokines and chemokines. Films were developed from array membranes following incubation with supernatants from either Mφ cultures, MDA-MB-231 cells or the coculture. Positive controls are located at positions A1, A2, D12. Negative controls are located at positions A3, A4 and D11. This experiment was repeated once with similar results.