In vitro study on the schedule-dependency of the interaction between pemetrexed, gemcitabine and irradiation in non-small cell lung cancer and head and neck cancer cells

Table 2 Influence of 24 h MTA on the percentage cells in G₁, S and G₂/M phase.

	Condition	G₁phase			S phase			G₂/M phase
		Mean	SD		mean	SD		mean	SD
CAL-27	24 h 0 nM MTA	49.3	2.2		34.3	2.5		10.8	2.4
	24 h 50 nM MTA → 0 h	30.4	13.7		56.2	14.1		8.9	3.8
	24 h 50 nM MTA → 1 h	30.9	12.9		54.5	12.3	*	9.5	4.0
	24 h 50 nM MTA → 4 h	30.1	9.8	*	53.5	7.5	*	10.8	1.6
	24 h 50 nM MTA → 8 h	29.0	0.1	*	49.1	2.4	*	15.2	2.0
	24 h 50 nM MTA → 24 h	41.5	7.9		38.9	6.8		9.2	8.1
A549	24 h 0 nM MTA	49.5	3.1		37.1	1.3		11.3	0.8
	24 h 100 nM MTA → 0 h	23.3	6.1	*	66.5	6.2	*	7.2	1.5	*
	24 h 100 nM MTA → 1 h	25.4	4.2	*	65.8	4.6	*	5.4	1.1	*
	24 h 100 nM MTA → 4 h	23.1	5.5	*	68.0	6.3	*	7.9	2.9
	24 h 100 nM MTA → 8 h	16.4	0.4	*	71.3	1.5	*	8.5	2.1
	24 h 100 nM MTA → 24 h	57.1	18.8		38.2	0.8		12.4	0.9

Cell cycle analysis was performed 0, 1, 4, 8 or 24 h after drug wash out. *: p < 0.05 vs. untreated control

ISSN: 1471-2407