In vitro study on the schedule-dependency of the interaction between pemetrexed, gemcitabine and irradiation in non-small cell lung cancer and head and neck cancer cells

Wouters, An; Pauwels, Bea; Lardon, Filip; Pattyn, Greet GO; Lambrechts, Hilde AJ; Baay, Marc; Meijnders, Paul; Vermorken, Jan B

doi:10.1186/1471-2407-10-441

Table 3 IC₅₀ and combination index (CI) values for MTA - dFdC combinations in CAL-27 and A549 cells.

From: In vitro study on the schedule-dependency of the interaction between pemetrexed, gemcitabine and irradiation in non-small cell lung cancer and head and neck cancer cells

Condition	CAL-27
	IC₅₀(nM)	SD		CI	SD		IC₅₀(nM)	SD		CI	SD
24 h MTA → 24 h wash out	118.53	16.37					569.47	120.60
24 h wash out → 24 h MTA	198.25	14.51					1576.86	349.19
24 h MTA + 24 h 2 nM dFdC → 24 h wash out	147.44	18.68		1.038	0.378		1689.43	1289.82		1.003	0.352
24 h MTA + 24 h 4 nM dFdC → 24 h wash out	240.31	49.10	†	0.957	0.510		9120.46	1499.06	†	0.569	0.271	**
24 h MTA → 24 h 2 nM dFdC	146.31	12.08	†	0.943	0.403		452.53	177.35		0.632	0.213	**
24 h MTA → 24 h 4 nM dFdC	130.60	29.92		0.858	0.461	*	1069.95	1035.22		0.373	0.190	**
24 h 2 nM dFdC → 24 h MTA	132.00	14.59	†	0.779	0.341	*	1322.54	833.14		0.546	0.237	**
24 h 4 nM dFdC → 24 h MTA	172.68	42.09		0.739	0.373	*	6076.56	3648.50		0.384	0.154	**
24 h dFdC → 24 h wash out	4.35	0.39					3.64	0.27
24 h wash out → 24 h dFdC	5.87	0.17					5.30	0.20
24 h dFdC + 24 h 50 nM MTA → 24 h wash out	4.25	0.37		1.141	0.858
24 h dFdC + 24 h 100 nM MTA → 24 h wash out	5.82	0.68	†	1.201	0.843
24 h dFdC + 24 h 200 nM MTA → 24 h wash out							7.48	1.04	†	1.112	0.972
24 h dFdC + 24 h 500 nM MTA → 24 h wash out							19.40	11.06		1.167	0.967
24 h dFdC → 24 h 50 nM MTA	3.83	0.07		1.073	0.872
24 h dFdC → 24 h 100 nM MTA	3.61	0.20		1.179	1.071
24 h dFdC → 24 h 200 nM MTA							4.90	1.79		0.873	0.924	*
24 h dFdC → 24 h 500 nM MTA							7.08	1.87		0.921	0.942
24 h 50 nM MTA → 24 h dFdC	4.88	0.29	†	0.961	0.591
24 h 100 nM MTA → 24 h dFdC	5.89	0.83		1.158	0.870
24 h 200 nM MTA → 24 h dFdC							6.96	2.52		0.830	0.848	*
24 h 500 nM MTA → 24 h dFdC							14.81	10.54		0.838	0.806	*
1 h dFdC → 24 h wash out	478.76	149.90					533.46	197.64
24 h wash out → 1 h dFdC	495.55	130.97					569.36	96.66
1 h dFdC → 24 h 50 nM MTA	571.61	262.49		1.154	0.349
1 h dFdC → 24 h 100 nM MTA	386.35	92.33		0.980	0.185
1 h dFdC → 24 h 200 nM MTA							421.84	237.54		0.693	0.287	**
1 h dFdC → 24 h 500 nM MTA							407.32	187.49		0.570	0.265	**
24 h 50 nM MTA → 1 h dFdC	271.36	38.36		0.880	0.466	*
24 h 100 nM MTA → 1 h dFdC	166.12	16.64	†	0.803	0.413	*
24 h 200 nM MTA → 1 h dFdC							215.44	21.72	†	0.880	0.682	*
24 h 500 nM MTA → 1 h dFdC							217.14	24.80	†	0.681	0.494	**

†: p < 0.05 vs. IC₅₀ of the corresponding drug alone; *: moderate synergism (0.7<CI<0.9); **: synergism (CI<0.7).

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ISSN: 1471-2407

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