Figure 3

(A) RT-PCR and western blotting results showing CIAPIN1 transcripts and protein in CRC cell lines. β-Actin was used as an internal control. (B) Electron microscopy of colon cancer cells during the process of induced differentiation. (a) Undifferentiated colon cells; (b-f) Changes in ultra-microstructure during colon cell differentiation. There were special conformations such as cryptate structures, tight junctions and macula adherens between cells, as well as polarisation of microvilli in differentiated HT29 and SW480 cells. (C) AP level during induced cell differentiation in two different colon cancer cell lines. AP levels in HT29 and SW480 were measured from the 1st to the 5th day during the induced differentiation process and compared with untreated cells (day 0). (D) RT-PCR and Western blotting results showing CIAPIN1 transcripts and protein changes during HT29 and SW480 cell line differentiation from the 1st to 5th day of induced differentiation compared with untreated cells (day 0). β-Actin was used as an internal control.