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Figure 2 | BMC Cancer

Figure 2

From: 17-Allylamino-17-demethoxygeldanamycin induces downregulation of critical Hsp90 protein clients and results in cell cycle arrest and apoptosis of human urinary bladder cancer cells

Figure 2

17-AAG-induced G1 cell cycle block is due to downregulation of specific Hsp90 clients. (A) Detection of Cdk4 protein (Western blotting) and Cyclin D1 gene (RT-PCR), both critically implicated in the G1 to S phase transition, over a 24-hours treatment period of human bladder cancer cells with 17-AAG. The mRNA expression levels of the internal control gene GAPDH were also quantified by an RT-PCR approach. (B) Western blotting of the cell cycle regulatory proteins pRb and E2F1, after exposure of RT4, RT112 and T24 cells to different 17-AAG doses. Immunoblotting and RT-PCR experiments were repeated three times.

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BMC Cancer

ISSN: 1471-2407

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