Figure 7

17-AAG administration results in functional attenuation of Akt signaling in bladder cancer cells. Inhibition of Hsp90 leads to prominent downregulation of the Akt-dependent signaling pathway, presenting with pronounced decrease of constitutively phosphorylated, and therefore activated, IGF-I receptor, Akt kinase and downstream signaling components, such as members of the IKK family (IKKα and IKKβ). All experiments were repeated three times.