Figure 5

Co-location of GSK-3β and PMS2 production in HeLa cells after GSK-3β transfection, as indicated by indirect immunofluorescence staining. Representative results for GSK-3β and PMS2 immunostaining were presented (200×). Immunostaining of GSK-3β was cytoplasmic and partly nuclear in HeLa cells, while PMS2 was mainly located in the nucleus. Transfection of GSK-3β WT and GSK-3β CA but not GSK-3β KD increased the intensity of PMS2 expression in HeLa cells, compared to the control. GSK-3β was labeled with Texas Red-conjugated secondary antibody, while PMS2 was labeled with FITC-conjugated secondary antibody. The right lane indicated co-location of GSK-3β and PMS2 in the merged image in HeLa cells.