Figure 6

PMS2 enhanced cisplatin-induced apoptosis in HeLa cells indicated by flow cytometric analysis. HeLa cells were transfected with PMS2 plasmid (2 μg/mL) and followed by treatment with cisplatin for 0-36 h. (A) PMS2 production was demonstrated to be elevated in HeLa cells after transfection for 0-36 h at a dose of 2 μg/ml. Representative results of western blot were shown. (B) PMS2 transfection (24 h) significantly enhanced cisplatin-induced apoptosis in HeLa cells. Annexin⁺/PI⁺ cells indicate late apoptosis cells and Annexin⁺/PI^- cells indicate early apoptosis cells. Representative results of apoptosis assay using the AnnexinV-FITC Apoptosis Detection Kit as described in Method Section was shown. The results are representative of two independent experiments. * P < 0.05 compared to the control cells.