Figure 3

Western blot analysis of VLDLR II expression from normal and tumor samples. 100 μg of total protein extracted from tumor tissues and matched adjacent noncancerous tissues were tested by Western blot. A-C: Some representative samples from gastric, breast, and lung cancer patients (8 for each), indicate as N and T for normal and tumor samples. VLDLR II expression levels are higher in gastric and breast tumor tissues compared with matched adjacent noncancerous tissues (A, B). In lung tumor tissues, VLDLR II is either high or similar as compared with normal tissues (C). The same filter membranes were probed with β-actin. The bands were densitometrically scanned and referred to β-actin as internal control. The box graphs are shown the statistical analysis with relative protein expression levels of VLDLR II in tissues from gastric, breast, and lung cancer patients (52, 46, and 51, respectively). Abbreviations: N, normal tissue; T, tumor tissue.