Figure 4

Impact of ATF3 down-regulation on subcutaneous tumor growth in vivo. The effects of ATF3 down-regulation on tumor growth were investigated in a subcutaneous tumor model. A) ATF3-shRNA-transfected HCT116 cells showed an accelerated in vivo growth rate, as compared to Luc-shRNA HCT116 cells (*P < 0.01). B) Changes in tumor growth rate were also reflected by final tumor weights at day 28 (*P < 0.01). C) CD31-staining demonstrated increased vascularisation in tumors of the ATF3-shRNA-transfected group, as compared to controls (*P < 0.05). Bars: mean ± SEM