Figure 6

Two examples of disproving antigens: aldolase C as the antigen in spot number 69b (a) and LMAN2 as the antigen in spots number 62a and 74a (b). A protein assigned to the immunoreactive spot 69b was excised from a preparative gel and identified as aldolase C. Duplex Western blotting with a human serum and an antibody directed against aldolase C does not show overlapping signals (a). The immunoreactive protein spots 62a and 74a were excised from a preparative gel and both identified as LMAN2 (VIP36). A VIP36-specific antibody showed a complex staining pattern consisting of a chain of four protein tracks extended in the second dimension. The spots 62a and 74a reside within or very close to one of these tracks. The staining patterns of the human sera and the VIP36-specific antibody, however, do not correspond to each other (b).