Figure 1

ScFvs expression in SiHa cells by retroviral system. SiHa cells transduced with the scFv51-expressing retroviruses carrying nuclear localization signal (51N, top panel, right) or endoplasmic reticulum localization signal (51SD, bottom panel, right) were analyzed by immunofluorescence. The transduced cells were fixed 24 h post-transduction with 4% paraformaldehyde and the scFv localization was detected by a mouse anti-myc mAb followed by an anti-mouse FITC-conjugated mAb, as a secondary antibody. The nuclei of transduced cells, shown on the left in top and bottom panels, were stained with PI.