Figure 5

Mapping of the scFv 43M2 and 51 epitopes on E7 protein by Surface Plasmon Resonance. Panel A: the sensorgram shows the relative binding of the two scFvs to the recombinant 16E7 immobilized on the sensor chip. The scFv 51 at saturating concentration was injected followed by scFv43M2. The curves show that scFv 43M2 and 51 have distinct epitope binding sites on E7. Panel B, top: the scFv 43M2 binding curves in the sensorgram were obtained by injecting scFv43M2 at a constant concentration (200 nM) following the binding of GST-pRb injected over the E7 sensor chip at different concentrations in the 0-400 nM range. Panel B, bottom: the Response Unit (RU) values for scFv 43M2 (dashed line) as a function of GST-pRb concentrations were measured 20 s after the end of each injection. The results were elaborated with KaleidaGraph Version 4.0.