Adaptation to HIF-1 deficiency by upregulation of the AMP/ATP ratio and phosphofructokinase activation in hepatomas

Table 1 Differentially expressed glycolytic and related proteins in Hepa-1 c4 and WT tumours.

Spot number	Protein Name	c4/WT Ratio	P values
47	Triose-phosphate isomerase (EC 5.3.1.1).	-1.46	0.0005
42	Glyceraldehyde-3-phosphatedehydrogenase	-1.35	0.0530
	(EC1.2.1.12).
52	Phosphoglycerate kinase (EC 2.7.2.3).	-1.89	0.0005
41	Phosphoglycerate mutase (EC 5.4.2.1).	-1.44	0.0005
49	Fructose bisphosphate Aldolase A (EC 4.1.2.13).	-2.05	0.0005
57	Enolase (EC 4.2.1.11).	-1.35	0.0005
60	Pyruvate kinase (EC 2.7.1.40).	-1.69	0.0005
59	D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95).	-2.39	0.0005
73	L-Lactate dehydrogenase A (EC 1.1.1.27).	-1.25	0.0140

Differences in 2D gels between the Hepa-1 c4 and WT tumours.
The c4/WT ratios of proteins are selected as differences in 2D gels between the Hepa-1 c4 (n = 3) and WT (n = 3) tumours, each one with a technical replicate using reciprocal labeling. The software produces 'fold' differences and P-values (t-test). The negative ratios indicate less in the Hepa-1 c4 than WT. See Additional File for list (Table S1) of all the protein changes.

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