Adaptation to HIF-1 deficiency by upregulation of the AMP/ATP ratio and phosphofructokinase activation in hepatomas

Table 2 Enzyme activity in Hepa-1c4 and WT cells and tumours

nmol/min/mg protein	c4 cells	c4 tumour	WT cells	WT tumour	P value
LDH	1640 ± 511	861 ± 198	2373 ± 421	1269 ± 133	>0.1
PK	326 ± 4	177 ± 40	470 ± 15	292 ± 12	<0.05*
PFK	19.1 ± 2.0	15.5 ± 1.5	20.0 ± 1.5	17.8 ± 1.4	>0.1

The activities are expressed as mean ± sem (n = 3) for cultured cells and tumours. The assays were performed under optimal conditions (for details see Methods).* denotes difference between c4 and WT.

ISSN: 1471-2407