Figure 5
Immuno-histochemical analysis of SW480 tumors in the nude mice treated with PL. The nude mice were inoculated with SW480 cells (5 × 106 cells/100 μL) and administered daily i.v. injections of PL (100 μg/100 μL of saline) or normal saline for 14 d after tumor cell implantation. Two weeks after the implantation, tumors were removed, and histological sections of the tumors from saline- or PL-treated mice were processed for immuno-histochemical staining and quantified for proliferation (Ki-67), apoptosis (TUNEL), and angiogenesis (CD31). A, Immuno-histochemical staining for proliferation, apoptosis, and angiogenesis. B, Quantification of proliferation, apoptosis, and angiogenesis index. The quantitative data in each case represent the mean ± SD of 8 animals in each group as detailed in section Methods. C, SW480 tumor tissue samples were immuno-histochemically stained for β-catenin immuno-reactivity (left) and quantified (right) as detailed in Methods. Quantitative data in each case represent the mean ± SD of 8 animals in each group. D, Expression of β-catenin and cyclin D1 in SW480 tumors. Total protein was prepared from saline- or PL-treated tumor tissues and electrophoresed in an SDS-PAGE gel followed by immuno-blotting for β-catenin and cyclin D1. The blots shown are representative of three independent experiments with similar results.