Figure 6

TGFβ regulates Snail1 and Nanog through Smad signaling. Epithelial cells were transfected with pcDNA control, Smad7 or ΔSmad3 and treated with TGFβ. (A) Relative Snail1 mRNA expression of epithelial cells. One-way ANOVA with Tukeys posthoc test was performed. Data shown as mean ± SEM of triplicates, *p < 0.01. (B) Western blot of Snail1 and β-actin. Data represent two independent experiments. (C) Epithelial cells were transfected with Nanog-Luc plasmid and treated with TGFβ. Luciferase activity was normalized to Renilla control. Data are shown as mean ± SEM of triplicates, *p < 0.05. (D) Relative Nanog luciferase activity after 24 hours of TGFβ stimulation. Data are shown as mean ± SEM of triplicates, *p < 0.05.