Figure 4

Eradication of human cancer cells with high occurrence of extra-centrosomes by the phenanthridine PJ-34. Top: Colon cancer cells DLD-1, lung cancer cells H1299, ovarian cancer cells HeyA8 and pancreatic cancer cells PANC-1 were examined under light microscope in randomly selected fields (0.02-0.03 mm² per field). The presented cells were either untreated (control) or incubated for 96 hours with PJ-34 applied once at 24 hours after seeding at the indicated concentrations. These are representative results of experiments that were repeated three times for each cell line. Bottom: Changes in cells' viability (total viable cells) were quantified by luminescent detection of ATP levels (% ATP level in cells treated with PJ-34 relative to ATP level measured in untreated, control cells) in each of the indicated cancer cell-lines. cells were incubated for 72 hours (grey line) and for 96 hours (black line) with PJ-34 applied once at 24 hours after seeding at the indicated concentrations (Methods). The mean values and standard deviations of 4 measurements in each cell-line in 3 different experiments are presented.