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Figure 2 | BMC Cancer

Figure 2

From: The HOPE fixation technique - a promising alternative to common prostate cancer biobanking approaches

Figure 2

DNA integrity of FFPE, HOPE, and fresh-frozen tissues. Control gene PCR for the assessment of amplification and integrity of DNA samples obtained from FFPE, HOPE, and fresh frozen tissues. Gel electrophoresis of five control gene exon PCR products ranging from 100 to 600 bp. a. FFPE derived DNA samples. Clear bands were observed for 4 control genes. The 600 bp band was visible only in lane 6, suggesting a certain amount of DNA degradation. b. HOPE derived DNA samples. Clear bands were observed for all 5 control genes. Results were comparable to the DNA extracted from fresh frozen samples, suggesting good DNA integrity. c. Fresh frozen derived DNA samples. Clear bands were observed for all 5 control genes suggesting good DNA integrity, as expected.

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BMC Cancer

ISSN: 1471-2407

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