Figure 5
From: Multimodal microscopy for automated histologic analysis of prostate cancer
Nucleus Detection. Smoothing and adaptive histogram equalization are performed to alleviate variability in H&E stained image and to obtain better contrast. "RG - B" conversion followed by thresholding characterizes the areas where nuclei exist. Morphological closing operation is performed to fill holes and gaps within nuclei, and a watershed algorithm segments each individual nuclei. The segmented nuclei are constrained by their shape, size, and average intensity and epithelial cell classification (green pixels) provided by the overlaid IR image.