Figure 2
From: Correlation between Slug transcription factor and miR-221 in MDA-MB-231 breast cancer cells
Effect of Slug knockdown on cell cycle and viability. MDA-MB-231 cells were transfected with si-Slug molecule and collected 72 hours after transfection. (A) Viability was determined by double staining assay with Calcein-AM and propidium iodide. Fluorescence photomicrographs (4X magnification) are representative merged images showing the presence of green fluorescence (calcein-AM)-labelled live cells and the absence of red fluorescence (PI)-labelled dead cells. (B) Cells were subjected to fluorescence-activated cell sorting analysis, and the relative G0/G1, S, and G2/M compartments calculated. Percentages of cells in each compartment are means of two independent experiments.