Figure 5

Effects of SPARC knockdown on cell growth, colony formation and apoptosis. (A) Cell proliferations of SPARC shRNA infected cells, control shRNA infected cells and non-infected cells as examined by MTT assay. (B) Anchorage independent growth of SPARC shRNA infected cells, control shRNA infected cells and non-infected cells as measured by soft agar colony formation assay. (C) Cell-cycle distributions of SPARC shRNA infected cells and control shRNA infected cells as measured by flow cytometry. (D) Cell apoptosis of SPARC shRNA infected cells, control shRNA infected cells and non-infected cells as measured by Annexin V-PI assays. *P<0.05 versus control.