Figure 2
From: Wnt5a promotes ewing sarcoma cell migration through upregulating CXCR4 expression
Upregulation of CXCR4 by Wnt5a in ES cells. (A) and (B) Level of CXCR4 mRNA increased in A-673 and RD-ES after treatment with rWnt5a, *P<0.01, vs Control (treated with PBS). (C) and (D) Wnt5a expression was silenced in SK-N-MC and SK-ES-1 cells after transfection with Wnt5a shRNA. (E) and (F) Level of CXCR4 mRNA decreased in SK-N-MC and SK-ES-1 cells after transfection with Wnt5a shRNA, *P<0.01, vs Control and Control shRNA. Data are expressed as mean±SD, n=3.