Figure 3

TGFβ and BMP pathway activity and its effect on proliferation. A: TGFβ pathway activity assayed using CAGA-luc reporter assay. The Y-axis is in percentage of luciferase activity with untreated cells set to 100%. Cells were treated with three different concentrations of the TGFβ inhibitor SB-431542 or with TGFβ1, to stimulate the pathway B: Proliferation of chondrosarcoma cells is not affected by treatment with TGFβ inhibitor or stimulator in two chondrosarcoma cell lines, nor in C2C12 cells. C: BMP pathway assayed using BRE-luc reporter assay, shown as in A. Inhibition of BMP with three different concentrations of LDN-193189 or stimulation with BMP4. D: Proliferation of chondrosarcoma cells is not affected by manipulation of BMP pathway activity.