Figure 4

Effect of 0.5nM 1,25(OH) ₂ D ₃ on expression of CYP24A1, CD14, CA2, DPP4, ILRL1 and BMP6 in cancer infiltrating fibroblasts and mammary epithelial cells. Cancer infiltrating fibroblasts (n=5) and breast derived cell lines (left to right: HB4A, C5.4, SKBR3, MCF7, MDA-MB231) were cultured in the presence or absence of 0.5nM 1,25(OH)₂D₃ for 24 h. Three independent assays for each cell line were performed and the mean relative gene expression value was calculated. Gene expression was determined by RT-qPCR and relative expression is shown on the Y axis (which is in log scale for CYP24A1, CA2, CD14, DPP4 and IL1RL1). p values: Wilcoxon signed ranks test. White bar: control samples; Gray bars: 1,25(OH)₂D₃ treated samples.