Figure 6

Oxidative and nitrosative stress in lung tissues 16 weeks post radiation exposure. Mice on control diet (0% FLC) were exposed to a single fraction thoracic X-ray radiation therapy (13.5 Gy). Following XRT exposure, mice were switched to 10% FLC or 20% FLC diets that were initiated 24 hours post radiation exposure while control-fed mouse cohorts remained on 0% FLC diet throughout the course of the study. Lungs were harvested at 16 weeks post-XRT, and BAL performed followed by paraffin embedding and immunostaining for nitrotyrosine; brown staining (Panel A). Representative lung sections are shown In Panels a-b: representing 0% FLC, Panels c-d: representing 10% FLC and Panels e-f: representing 20% FLC. Sections were counterstained with methyl green. (Magnification 400X). Levels of thiobarbituric acid reactive substances (TBARS) in BAL samples were determined at 16 weeks post-XRT (Panel B). Data is represented as mean ± SEM. # p< 0.05 for 0% FLC vs. irradiated 0% FLC; *p< 0.01 for irradiated 0% FLC vs. irradiated 10% and 20% FLC.