Figure 4

siRNA inhibited UBE2C expression in NPC cells and consequently resulted in attenuated cell proliferation. (A) C666-1 cells were transfected with si-UBE2C or si-Control or without siRNA (MOCK). Forty-eight hours later, UBE2C expression was assessed by PCR and immunofluorescence using TRITC-conjugated IgG (H+L). (B) Western blotting analysis the UBE2C protein expression in various cell lines post tranfection of siRNAs, the β-actin was used as loading controls. (C) Twenty-four and 48 h after transfection, CCK-8 assays were used to analyze the proliferation of various types of NPC cells. Values of optical density (OD) were obtained by the absorbance at the dual wavelengths 450/630 nM, and the results indicating the cell viability were plotted as the percentage over controls (MOCK cells). * P<0.05, ** P<0.01 vs. mock or si-Control–treated groups.