Table 1 Primers and oligonucleotides used in this work
Name | Sequence (5′-3′) |
|---|---|
Pri-miR-302b-S | 5′-AATTCGCTCCCTTCAACTTTAACATGGAAGTGCTTTCTGTGACTTTAAAAGTAAGTGCTTCCATGTTTTAGTAGGAGTA-3′ |
Pri-miR-302b-A | 5′-AGCTTACTCCTACTAAAACATGGAAGCACTTACTTTTAAAGTCACAGAAAGCACTTCCATGTTAAAGTTGAAGGGAGCG-3′ |
EGFR 3′UTR-S | 5′-CAAGAAGCTTGCTGGTAGCACTTGC- 3′ |
EGFR 3′UTR-A | 5′-TCGAGCAAGTGCTACCAGCAAGCTTCTTGAGCT- 3′. |
EGFR 3′UTR-MS | 5′-CAAGAAGCTTGCTGGCAGCGTTTGC-3′ |
EGFR 3′UTR-MA | 5′-TCGAGCAAACGCTGCCAGCAAGCTTCTTGAGCT-3′ |
siRNA-ctrl-S | 5′-ACCGAACGTGTCACGT-3′ |
siRNA-ctrl-A | 5′-ACGTGACACGTTCGGAGAATT-3′ |
siEGFR-S | 5′-AACACAGTGGAGCGAATTCCT-3′ |
siEGFR-A | 5′-AGGAATTCGCTCCACTGTGTT-3′ |
miR-302b RT | 5′-TGCTTAAGTGCTTCCATGTT-3′ |
miR-302b-F | 5′-ATCCAGTGCGTGTCGTG-3′ |
miR-302b-R | 5′-TGCTTAAGTGCTTCCATGTT-3′ |
Inhibitor-ctrl | 5′-TGACTGTACTGACTCGACTG-3′ |
MiR-302b inhibitor | 5′ -TGATTTTGTACCTTCTGGAAT-3 |
EGFR-F | 5′-GCCTTGACTGAGGACAGCA-3′ |
EGFR-R | 5′-TTTGGGAACGGACTGGTTTA-3′ |
β-actin-F | 5′-CGGGAAGCTTGTCATCAATGG-3′ |
β-actin-R | 5′-GGCAGTGATGGCATGGACTG-3′ |
U6 RT | 5′-GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAAAATATGG-3′ |
U6-F | 5′-TGCGGGTGCTCGCTTCGGCAGC-3′ |
U6-R | 5′ CCAGTGCAGGGTCCGAGGT 3′ |