Figure 3

MT1G induced thyroid cancer cell apoptosis. K1 and FTC133 cells were transiently transfected with pEGFP-N1-MT1G or empty vector. After 48 h post-transfection, apoptotic cells were determined by Hoechst 33342 staining and observed under a fluorescent microscope. Selective apoptotic nuclei showing characteristics of apoptosis, including chromatin margination, condensation, and fragmentation, were indicated by arrows. Relative percentage of apoptotic cells in the MT1G-transfected and empty vector-transfected groups was presented in right panel. *, P <0.05.