Figure 2
From: Selective apoptosis induction in MCF-7 cell line by truncated minimal functional region of Apoptin
Double digestion analysis of constructed Apoptin and truncated Apoptin. The insert in plasmids were analysed for the presence of the truncated inserts by double digestion with NcoI and XhoI. Lane 1: wild-type VP3 gene with length 366 bp, lane 2: truncated VP3 gene pMBP-VP3A1-69N1N2 with length 261 bp, lane 3: truncated VP3 gene pMBP-VP3A1-46N1N2 with length 191 bp, lane 4: truncated VP3 gene pMBP-VP3A1-31N1N2 with length 150 bp, Lane 5: truncated VP3 gene pMBP-VP3A32-69N1N2 with length 174 bp and lane 6: truncated VP3 gene pMBP-VP3A32-62N1N2 with length 150 bp. Lane M: 50 bp DNA Ladder (New England Biolabs, USA).