Figure 2

MLCK is required for cell migration and invasion in response to LPA, HGF and EGF. MDA-MB-231 cells were allowed to migrate across a collagen-coated transwell (A) or invade through Matrigel (B) toward LPA, HGF or EGF for 3 hours in the presence or absence of the MLCK inhibitor, ML-7 (inhib.), as noted. The numbers of cells migrated or invaded per mm² were graphed with their standard deviations for triplicate wells within a single experiment. For simplicity of presentation, statistically significant differences between stimulated and stimulated plus inhibitor-treated cell conditions only were determined using a t-test (*p < 0.02). Data are representative of a minimum of three independent assays for each condition.