Figure 5

A: Bar chart of ribonucleoprotein-immunoprecipitation (RIP) performed on A2780 cells in normoglycemia and after 48 hours of hypoglycemia with anti-HuR antibody and with an irrelevant anti IgG antibody. The qPCR analysis of miR-200c present in the RIP complexes was quantified by normalization with the corresponding not immunoprecipitated extracts. Data were shown as relative RIP, as IgG (+) =1. B-C: Bar charts of RIP performed in A2780, A2780-pUSE, A2780-200c-14, Hey and OVCAR-3 cells as indicated with anti-HuR antibody and with an irrelevant anti IgG antibody. Statistical comparison was performed only among isogenic cells. Asterisk mark the significant differences as compared to A2780 parental cells while § marks the difference as compared to pUSE cells. For both significance was set at a P<0.05. The qPCR analysis of TUBB3 mRNA, HPRT mRNA, miR-200c and miR-200a present in the RIP complexes was quantified by normalization with anti-IgG RIP and the corresponding not immunoprecipitated extracts. Values were normalized for the levels obtained in A2780 cells (=1).