Figure 5

Candidate hits in resistant clones. A) Cluster analysis of IMR32 resistant clones. X-axis indicates colonies and y-axis indicates insertion sites. Colonies within a cluster have same insertions and are likely derived from one founder clone. Insertions are in either same (red) or opposite (blue) orientations of a gene. B) Paclitaxel sensitivity curve of IMR32 transfected with a control (CTL) or ABCB1 cDNA plasmid (ABCB1). Cell survival was measured by CellTiter-Glo assay. C) Western blot showing ABCB1 overexpression in IMR32 cells transfected with pCMV-ABCB1 plasmid. CTL, cells transfected with a control pCMV plasmid; ABCB1, cells transfected with pCMV-ABCB1 cDNA plasmid. ABCB1 was shown as bands at 150kD. Actin was used as loading control. D) PB insertions in MEIS1 gene. The direction of gene is drawn from left to right, with yellow squares indicating exons. Forward strand insertion sites are drawn as green triangles and reverse as red triangles. Scale is drawn as per kb. E) Paclitaxel sensitivity profile in a panel of cancer cell lines. Cell lines are either divided to two groups by median ABCB1 or MEIS1 mRNA levels, n=143, (first and second graphs), or first divided by median ABCB1 levels and then by median MEIS1 mRNA levels, n=72 (ABCB1 Low) and 71 (ABCB1 High). Red bars indicate median IC50.