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Figure 3 | BMC Cancer

Figure 3

From: 3D pancreatic carcinoma spheroids induce a matrix-rich, chemoresistant phenotype offering a better model for drug testing

Figure 3

Metabolical and physiological analyses comparing 2D with 3D culture. [A] Energy metabolism: lactate accumulation measurements in 3D compared to 2D culture. Data are expressed as ratio μmoles lactate/ μg protein. [B] Western immunoblotting showing HIF1a protein expression both in 2D and 3D cultures at day 4 and 7. [C] mRNA expression of target genes downstream HIF1a in 2D and 3D cultures. Real Time-PCR data are calculated as expression ratio 3D/2D. A representative experiment out of three is shown. [D] Western blotting shows Glut1 protein expression both in 2D and 3D cultures on day 4 and 7. GAPDH is used as loading control.

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BMC Cancer

ISSN: 1471-2407

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